Method: Three different matcha drinks made with the same grade matcha, water temperature, and water to powder ratio. Only difference is the amount of time each powder was exposed to air before being mixed with water. From top to bottom, the first bowl was made immediately as the powder was sifted into the bowl. The second bowl was exposed to air for 5 hours before the powder and water were mixed. And the third bowl was exposed for 12+ hours.
Results: The color difference was slight. However, the third bowl tasted 5x times more bitter than the first. The second bowl also tasted more bitter than the first, but nowhere near as bitter as the third.
Conclusion: The longer matcha powder is exposed to air, the more bitter will the whisked matcha taste. Dark and airtight containers will help preserve true matcha flavor.